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Development of a semi-automatic system for pollen recognition
Authors:Alain Boucher  Pablo J Hidalgo  Monique Thonnat  Jordina Belmonte  Carmen Galan  Pierre Bonton  Régis Tomczak
Institution:(1) INRIA, Sophia-Antipolis, 2004 route des Lucioles, B.P. 93, F-06902 Sophia-Antipolis Cedex, France;(2) Department of Plant Biology, University of Córdoba, Campus Universitario de Rabanales, 14071- Córdoba, Spain;(3) Unit of Botany, Autonomous University of Barcelona, 08193 Bellaterra (Cerdanyola del Vallés), Spain;(4) LASMEA, UMR 6602 du CNRS, Blaise Pascal University, F-63117 Aubière Cedex, France
Abstract:A semi-automatic system for pollen recognitionis studied for the european project ASTHMA. The goal of such a system is to provideaccurate pollen concentration measurements. This information can be used as well by thepalynologists, the clinicians or a forecastsystem to predict pollen dispersion. At first,our emphasis has been put on Cupressaceae, Olea, Poaceae and Urticaceae pollen types. The system is composed of two modules: pollengrain extraction and pollen grain recognition. In the first module, the pollen grains areobserved in light microscopy and are extractedautomatically from a pollen slide coloured withfuchsin and digitized in 3D. In the secondmodule, the pollen grain is analyzed forrecognition. To accomplish the recognition, itis necessary to work on 3D images and to usedetailed palynological knowledge. Thisknowledge describes the pollen types accordingto their main visible characteristerics and tothose which are important for recognition. Somepollen structures are identified like the porewith annulus in Poaceae, the reticulum in Oleaand similar pollen types or the cytoplasm inCupressaceae. The preliminary results show therecognition of some pollen types, likeUrticaceae or Poaceae or some groups of pollentypes, like reticulate group.
Keywords:aerobiology  automatic pollen counting  automatic pollen recognition  colour image analysis  3D study  light microscopy  pollen morphology
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