Improved ethanol tolerance in <Emphasis Type="Italic">Escherichia coli</Emphasis> by changing the cellular fatty acids composition through genetic manipulation |
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Authors: | Lian Hua Luo Pil-Soo Seo Jeong-Woo Seo Sun-Yeon Heo Dae-Hyuk Kim Chul Ho Kim |
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Institution: | (1) Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, 580-185 Jeonbuk, South Korea;(2) Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 561-756 Chonbuk, South Korea; |
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Abstract: | To investigate the effect of cellular fatty acids composition on ethanol tolerance in Escherichia coli, we overexpressed either des, encoding fatty acid desaturase from Bacillus subtilis, or fabA, encoding β-hydroxydecanoyl thio-ester dehydrase from E. coli, or both genes together, into E. coli. Recombinant E. coli harboring fabA had elevated tolerance against ethanol compared to wild type strain. In contrast, des decreased resistance to ethanol. Co-expression of both genes together complemented ethanol tolerance of E. coli. This result indicates how to engineer bacterial strains to be resistant to higher concentrations of ethanol. |
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