Content of carotenoids during ageing and senescence of tobacco leaves with genetically modulated life-span |
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Authors: | D Procházková D Haisel N Wilhelmová |
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Institution: | (1) Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oun-dong, Yusong-gu, Daejeon, 305-806, Korea;(2) Bioresources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Korea;(3) Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Korea;(4) Department of Biology, Chungnam National University, Daejeon, 305-764, Korea; |
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Abstract: | We exploited leaves of tobacco (Nicotiana tabacum L., cv. Wisconsin 38) with introduced chimeric construct consisting of SAG12 promoter fused with ipt gene for cytokinin synthesis and therefore prolonged life-span. As a control we used its wild type. In 12-week-old plants,
the first leaves of control plants showed senescence symptoms at the time of sampling. Carotenoid content decreased with increasing
leaf age both in control and in transgenic plants. On the other hand, the first leaves of transgenic plants demonstrated better
antioxidant capacity represented by carotenoids compared to the leaves of control plants of the same age. They stayed still
green at this age. |
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Keywords: | |
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