In vitro phosphorylation ofEscherichia coli isocitrate lyase |
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Authors: | Eugene F Robertson Jeffrey C Hoyt Dr Henry C Reeves |
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Institution: | (1) Department of Microbiology, Arizona State University, 85287 Tempe, Arizona, USA |
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Abstract: | The in vitro phosphorylation of isocitrate lyase was demonstrated in partially purified sonic extracts ofEscherichia coli. Extracts were incubated with gamma32P]-ATP and subsequently analyzed by two-dimensional polyacrylamide gel electrophoresis. Isocitrate lyase was determined to be phosphorylated by autoradiography and Western blot analyses of the gels. Purified isocitrate lyase comigrates with the phosphorylated form of the enzyme; this suggests that the enzyme may become catalytically active concomitant with phosphorylation. |
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