Synaptonemal complex analysis in spermatocytes of diploid and triploid Chinese shrimp Fenneropenaeus chinensis |
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Authors: | Xie Yusu Li Fuhua Zhang Chengsong Yu Kuijie Xiang Jianhai |
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Affiliation: | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China. |
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Abstract: | A modified surface spreading technique for synaptonemal complex (SC) analysis was tested to assess the process of chromosome synapsis in spermatocytes of diploid and induced triploid Fenneropenaeus chinensis. Spermatocytes of diploid shrimp showed typical morphological characteristics of eukaryote SC, with complete synapsis of bivalents. No recognizable bivalent associated with sex chromosomes was observed in spermatocytes of diploid shrimp. However, differences in morphology of SC, including unsynapsed univalents, bivalents, totally paired trivalents with non-homologous synapsis, partner switches and triple synapsis were identified at early pachytene stage of triploid spermatocytes. Triple synapsis was especially common at late pachytene stage in spermatocytes of triploid shrimp. The observed abnormal synapsis behavior of chromosomes in spermatocytes indicated that triploid male shrimp may find it difficult to develop normal haploid sperm. |
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Keywords: | Synaptonemal complex Fenneropenaeus chinensis Spermatocytes Diploid Triploid |
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