Culture of endothelial cells by transfection with plasmid harboring vascular endothelial growth factor |
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Authors: | Sungjae Chang Insook Sohn Inchul Park Youngsook Sohn Seokil Hong Taeboo Choe |
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Institution: | (1) Department of Microbial Engineering, Konkuk University, 93-1, Mojin-dong, Kwangjin-gu, 143-701 Seoul, Korea;(2) Department of Gynecology, Konkuk University, 93-1, Mojin-dong, Kwangjin-gu, 143-701 Seoul, Korea;(3) Laboratory of Cell Biology, Korea Cancer Center Hospital, 139-706 Seoul, Korea |
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Abstract: | Vascular endothelial cells (ECs) are usually difficult to culture in a large scale because of their complicated requirements
for cell growth. As the vascular endothelial growth factor (VEGF) is a key growth factor in the EC culture, we transfected
human umbilical vein endothelial cells (HUVEC) using a plasmid containing VEGF gene and let them grow in a culture medium
eliminated an important supplement, endothelail cell growth supplement (ECGS). The expression of VEGF by HUVEC tansfected
with VEGF gene was not enough to stimulate the growth of HUVEC, only 40% of maximum cell density obtainable in the presence
of ECGS., However, when the culture medium was supplied with 2.5 ng/mL of basic fibroblast growth factor (bFGF), a synergistic
effect of VEGF and bFGF was observed. In this case, the final cell density was recovered up to about 78% of maxium value. |
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Keywords: | endothelial cell culture vascular endothelial growth factor transfection autocrine |
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