Early pregnancy affects the expression of toll-like receptor pathway in ovine thymus |
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| Affiliation: | 1. Department of Oral and Maxillofacial Surgery, University Hospital of Salzburg, Salzburg, Austria;2. Department of Orthopaedic and Trauma Surgery, University Hospital of Salzburg, Salzburg, Austria;1. Department of Veterinary Gynaecology and Obstetrics, Hisar, 125 004, Haryana, India;2. Veterinary Clinical Complex, Hisar, 125 004, Haryana, India;3. Department of Animal Biotechnology, Hisar, 125 004, Haryana, India;4. ICAR-Central Institute for Research on Buffalo, Hisar, 125 004, Haryana, India;5. Department of Animal Genetics and Breeding, Hisar, 125 004, Haryana, India;6. Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125 004, Haryana, India;1. Institute of Veterinary Biochemistry, Freie Universität Berlin, Berlin, Germany;2. University Clinic for Ruminants, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria;3. Institute of Microbiology, Functional Microbiology, Department for Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria;1. Department of Animal Science, College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, China;2. Hebei Province Engineering Technology Research Center of Dairy Cattle Breeding, Shijiazhuang 050051, China;1. Johns Hopkins University, United States;2. University of Michigan, United States;3. Janelia Research Campus, United States |
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| Abstract: | Toll-like receptors (TLRs) participates in regulation of the maternal immune tolerance during pregnancy, and the thymus is critical for the adaptive immune system. This study hypothesized whether early pregnancy affected the expression of toll-like receptor pathway in the thymus of ewes. In this study, expression of TLRs, tumor necrosis factor receptor associated factor 6 (TRAF6), interleukin 1 receptor associated kinase 1 (IRAK1) and myeloid differentiation primary response gene 88 (MyD88) was detected in maternal thymus during early pregnancy in sheep. Ovine thymuses were collected on day 16 of the estrous cycle, and days 13, 16 and 25 of pregnancy, and expression of TLR members was analyzed by real-time quantitative PCR, western blot and immunohistochemistry analysis. The results revealed that there were decreases in the expression of the mRNA and proteins of TLR2, IRAK1, TRAF6 and MyD88, but increase in TLR5 mRNA and protein. Furthermore, expression of TLR3 and TLR4 proteins peaked at days 13 and 16 of gestation, and MyD88 protein was located in the epithelial reticular cells and thymic corpuscles. In summary, TLR signaling is implicated in regulation of maternal thymic immune, which may be via downregulation of TLR2, IRAK1, TRAF6 and MyD88 during early pregnancy in sheep. |
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| Keywords: | Thymus Pregnancy Toll-like receptors Sheep |
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