Hematopoietic Stem/Progenitor Cell Sources to Generate Reticulocytes for Plasmodium vivax Culture |
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| Authors: | Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie |
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| Institution: | 1. Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.; 2. Department of development and regeneration, Stem Cell Institute, Leuven, Belgium.; 3. Unit of Veterinary Protozoology, Institute of Tropical Medicine, Antwerp, Belgium.; 4. Medical Research Council Unit, Fajara, The Gambia.; Instituto de Higiene e Medicina Tropical, Portugal, |
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| Abstract: | The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1–2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies. |
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