Induction of pistil-like structures in suspension-derived callus cultures of wheat (Triticum aestivum)

In the following a method for the induction of pistil-like structures in wheat suspension cultures (Triticum aestivum L.) is described. In young influorescences of plants, which were artificially infected with the wheat bunt fungi (Tilletia controversa), organogenic calli with pistil-like structures could be induced on loblolly pine medium + 3 mg/l 3,6-dichloro-2-methoxy benzoic acid. The yield of these structures in calli from a five-month-old suspension culture was up to 100 per gram of callus fresh mass.Abbreviations LM Loblolly pine medium - Dicamba 3,6-dichloro-2-methoxy benzoic acid