A protocol toward multiplication of the medicinal tree,Eucommia ulmoides oliver |
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Authors: | Li-Jing Chen Ta-Wei Hu Li-Chun Huang |
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Institution: | (1) Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China |
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Abstract: | Summary A protocol based on shoot cultures of 1-mo.-old seedlings was developed for rapid asexual multiplication of eucommia, the
source of an antihypertensive medicinal. The explant is an excised shoot tip, 3–5 mm tall. MS basal medium supplemented with
1 mg/liter BA is employed to establish primary cultures and subsequently multiply shoots. Shoots are subculturable on the
same medium and can be increased at a rate of 7.5 new shoots per 2-shoot sector every 3 wk. Rooting is achieved in a Gelrite
medium with the MS salts reduced to 1/3 strength and the BA replaced by 0.1 mg/liter NAA. The method is not directly applicable
to mature trees. Applicability will require explants from rejuvenated sources, possibly attainable by the method of repeated
grafting of shoot apices onto juvenile rootstocks, repeated subculturing of shoots, or culturing shoot apical meristems. |
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Keywords: | Eucommia ulmoides medicinal tree micropropagation tu-chung |
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