Thermodynamics of the charge recombination in photosystem II

The temperature dependence of the electric field-induced chlorophyll luminescence in photosystem II was studied in Tris-washed, osmotically swollen spinach chloroplasts (blebs). The system II reaction centers were brought in the state Z⁺P⁺-Q_A ^-Q_B ^- by preillumination and the charge recombination to the state Z⁺PQ_AQ_B ^- was measured at various temperatures and electrical field strengths. It was found that the activation enthalpy of this back reaction was 0.16 eV in the absence of an electrical field and diminished with increasing field strength. It is argued that this energy is the enthalpy difference between the states IQ_A ^- and I^-Q_A and accounts for about half of the free energy difference between these states. The redox state of Q_B does not influence this free energy difference within 150 s after the photoreduction of Q_A. The consequences for the interpretation of thermodynamic properties of Q_A are discussed.Abbreviations DCMU 3(3,4-dichlorophenyl)-1,1-dimethylurea - I intermediary electron acceptor - Mops 3-(N-morpholino)propanesulphonic acid - P (P₆₈₀) primary electron donor - PS II photosystem II - Q_A and Q_B first and second quinone electron acceptors - Tricine N-tris(hydroxymethyl)methylglycine - Tris tris-(hydroxymethyl)aminomethane - Z secondary electron donor Dedicated to Professor L.N.M. Duysens on the occasion of his retirement