Somatic embryogenesis and plant regeneration of Gladiolus (Gladiolus hort.) |
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Authors: | Barbara Stefaniak |
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Institution: | (1) Department of General Botany, Faculty of Biology, University of Pozna, 61-713 Pozna, Poland |
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Abstract: | Summary Friable embryogenic callus and somatic embryos of 4 Gladiolus cultivars were obtained on Murashige and Skoog (MS) medium with various concentration of auxins from the following explants: corm slices, young leaf bases and whole, intact plantlets. Somatic embryos transferred on MS hormone-free medium regenerated into plantlets. All plantlets obtained through embryogenesis did not differ phenotypically from the parental clones. The embryogenic friable callus has been maintained for over 2 years in culture and has retained a very high regeneration capacity.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- KIN
kinetin
- NAA
naphthaleneacetic acid
- MS
Murashige and Skoog Medium (1962)
- E
embryogenic callus
- NE
non-embryogenic callus |
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Keywords: | Gladiolus Plant regeneration Somatic embryogenesis |
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