A genetically engineered <Emphasis Type="Italic">Escherichia coli</Emphasis>, expressing the fusion protein of green fluorescent protein and carboxylesterase B1, can be easily detected in the environment following degradation of pesticide residues |
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| Authors: | Qin Li Rui Chen Wei Li Chuan-Ling Qiao Yi-Jun Wu |
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| Institution: | (1) Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, P.R. China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P.R. China |
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| Abstract: | Genetically engineered Escherichia coli, expressing the fusion protein of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), was successfully
constructed by cloning the genes into the pET-28b vector and then transforming E. coli BL21 (DE3). Expression of the fusion protein was induced in E. coli BL21 (DE3) which could then degrade environmental pesticides and could be easily detected using fluorescence spectrophotometry
or by the naked eye in daylight. |
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| Keywords: | Carboxylesterase B1 Escherichia coli Fusion expression Genetically engineered bacteria Green fluorescent protein |
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