| 用地高辛标记原位杂交技术定位黄鳝rRNA基因于二价染色体3q12─q24和7q14─q26 |
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| 引用本文： | 李奎,余其兴,毛勇,赵则春,樊连春. 用地高辛标记原位杂交技术定位黄鳝rRNA基因于二价染色体3q12─q24和7q14─q26[J]. 遗传学报, 1995, 0(2) |
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| 作者姓名： | 李奎 余其兴 毛勇 赵则春 樊连春 |
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| 作者单位： | 武汉大学生命科学学院遗传学系 |
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| 基金项目： | 国家自然科学基金,博士点科研基金 |
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| 摘 要： | 以地高辛标记重组质粒ＰＴＡ７１中所含的小麦ｒＲＮＡ基因作为探针，与ＥｃｏＲＩ酶切的黄鳝核基因组总ＤＮＡ经Ｓｏｕｔｈｅｒｎ杂交，呈现２条带，片段长度分别为１２．８ｋｂ和４．６ｋｂ。再运用染色体原位杂交技术及杂交后多重带显带技术，定位ｒＲＮＡ基因于黄鳝二价染色体３ｑ１２－ｑ２４和７ｑ１４－ｑ２６两个区间，其分布位点与硝酸银染色法结果相符。此外，还讨论了在黄鳝二价体上开展基因定位研究的突出优点。
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| 关 键 词： | 黄鳝，rRNA基因，二价染色体，原位杂交，Southern杂交 |
Localization of rRNA Genes of Rice -field Eels(Monopterus albus Zuiew) on the Bivalents 3q12-q24 and 7q14-q26 by Digoxigenin Labelled in situ Hybridization |
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| Li Kui, Yu Qixing, Mao Yong, Zhao Zechun,Fan Lianchun. Localization of rRNA Genes of Rice -field Eels(Monopterus albus Zuiew) on the Bivalents 3q12-q24 and 7q14-q26 by Digoxigenin Labelled in situ Hybridization[J]. Journal of Genetics and Genomics, 1995, 0(2) |
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| Authors: | Li Kui Yu Qixing Mao Yong Zhao Zechun Fan Lianchun |
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| Abstract: | Using the digoxigenin labelled wheat rDNA inserted into PTA71 as the probe,two bands,12.8kb and 4.6kb, have been shown to hybridize with total DNA of Rice-Field Eels digested with EcoRI by Southern blotting.Two rDNA locus have beenmapped on bivalents 3q12-q24 and 7q14-q26,respectively, by in situ hybridization combined with two multiple banding methods after hybridization.The results havebeen confirmed by Ag-NOR staining. The apparent advantages of mapping genes onbivalents have been discussed in detail.Received November 8, 1993. This project supported by State Eduction Commission Research Foun dation for Dectoral Disciplines and National Natural Science Foundation of China |
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| Keywords: | Monopterus albus Bivalents rDNA in situ hybridization Southernblotting |
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