| 大鼠肾脏和其它组织中磷酸酪氨酸蛋白的研究 |
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| 引用本文： | 张明志,张光毅,王迎伟,赵升皓.大鼠肾脏和其它组织中磷酸酪氨酸蛋白的研究[J].中国生物化学与分子生物学报,1993,9(2):213-218. |
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| 作者姓名： | 张明志 张光毅 王迎伟 赵升皓 |
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| 作者单位： | 徐州医学院生化教研室，徐州医学院生化教研室，徐州医学院生化教研室，徐州医学院生化教研室 徐州 221002，徐州 221002，徐州 221002，徐州 221002 |
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| 摘 要： | 本文采用P-tyr-BSA为免疫原免疫家无得抗血清。将纯化的IgG与HRP偶联,建立了P-tyr-Pr的ELISA法,并测定了正常大鼠肾脏等组织中P-tyr-Pr含量,其分布规律如下:上清中P-tyr-Pr含量高者,其颗粒部分则低,反之亦然;其中肾脏上清中含量远比其它组织(脾、肺、肝等)高。在此基础上,又研究了膜性肾炎大鼠肾脏P-tyr-Pr含量,发现其上清中的含量远远高于正常大鼠肾脏中的含量。
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| 关 键 词： | 磷酸酪氨酸蛋白 磷酸酪氨酸蛋白抗体 ELISA法 大鼠肾脏 Heymann膜性肾炎 |
| 收稿时间： | 1993-04-20 |
Studies on P-tyr-Pr in Rat Kidney and Other Tissues |
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| Zhang,Ming-zhi Zhang,Guang-yi Wang,Ying-wei Zhao,Sheng-hao.Studies on P-tyr-Pr in Rat Kidney and Other Tissues[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(2):213-218. |
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| Authors: | Zhang Ming-zhi Zhang Guang-yi Wang Ying-wei Zhao Sheng-hao |
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| Institution: | (Department of Biochemistry, Xuzhou Medical College, Xuzhou 221002 |
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| Abstract: | Antiserum for P-tyr-Pr was obtained by immunizing rabbit with P-tyr-BSA. IgG was purified and coupled to HRP. ELISA for P-tyr-Pr was established and the contents of P-tyr-Pr in rat tissues were detected. The characteristic of P-tyr-Pr distribution in rat tissues was as follows: the tissue with high P-tyr-Pr content in the supernatant contained low P-tyr-Pr in the particle part; while the tissue with low P-tyr-Pr content in the supernatant contained high P-tyr-Pr in the particle part. The content of P-tyr-Pr in the kidney supernatant was much higher than in supernatant of any other rat tissue. We further studied the content of P-tyr-Pr in membranous nephritis rat kidney and found that the content of P-tyr-Pr in its supernatant was much higher than that in normal rat kidney supernatant. |
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| Keywords: | P-tyr-Pr Antibody of P-tyr-Pr ELISA Rat kidney Heymann membranous nephritis |
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