Isolation,origin and properties of enucleate plant microplasts |
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Authors: | P C Bilkey M R Davey E C Cocking |
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Institution: | (1) Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, 7 2 RD Nottingham, NG, UK;(2) International Plant Research Inst. Inc., 853 Industrial Road, 94070 San Carlos, CA, USA |
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Abstract: | Summary Auxin-induced highly vacuolated thin-walled callus cells of several plant species, when ruptured, released large numbers of subcellular units most of which were enucleate. These enucleate microplasts are surrounded by an inner membrane of the cell, most probably derived from the tonoplast. When microplasts isolated fromSaintpaulia callus were cultured in a medium supplemented with growth substances they formed a thin wall and underwent budding. Microplasts could be useful for various studies in plant cell biology and for genetic manipulations. |
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Keywords: | Callus culture Enucleation Microplasts |
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