Effects of C/N ratio and trace elements on mycelial growth and exo-polysaccharide production of <Emphasis Type="Italic">Tricholoma matsutake</Emphasis> |
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| Authors: | Sung Su Kim Jong Seok Lee Jae Youl Cho Young Eon Kim Eock Kee Hong |
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| Institution: | (1) Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan;(2) New Zealand Institute for Crop and Food Research Limited, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand;(3) Unité Mixte de Recherche INRA-UHP Interactions Arbres/Micro-organismes, Institut National de la Recherche Agronomique, 54280 Champenoux, France |
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| Abstract: | Although its demand increased greatly due to the volatile strong flavor and bioactive molecules, little information has been
about the cultural characteristics of Tricholoma matsutake. In this study, we investigated the optimal medium composition of liquid culture with the goal of shortening the culture
period, and to maximize polysaccharide production and mycelial growth. From these experiments we found that the optimal medium
contained 40 g/L, glucose; 30 g/L, yeast extract; 1.5 g/L, KH2PO4; and 1 g/L MgSO4·7H2O. In flask culture, the maximum mycelial growth and polysaccharide production were 22.45 and 5.3 g/L, which were about 9
and 3 g/L higher than that at the basal medium, respectively. |
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