| Ghrelin对豚鼠胃窦平滑肌细胞钙离子浓度的影响及与NO关系研究 |
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| 引用本文： | 信芳杰,徐珞.Ghrelin对豚鼠胃窦平滑肌细胞钙离子浓度的影响及与NO关系研究[J].现代生物医学进展,2010,10(2). |
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| 作者姓名： | 信芳杰 徐珞 |
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| 作者单位： | 青岛大学医学院病理生理教研室,山东青岛,266021 |
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| 基金项目： | 国家自然科学基金项目(30470642和30670780); 山东省科技攻关项目(2008GG10002006); 山东省卫生厅项目(2007HZ026); 青岛市科技局项目(05-1-JC-93) |
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| 摘 要： | 目的:探讨Ghrelin对豚鼠胃窦平滑肌细胞内钙离子浓度的影响及其与一氧化氮(NO)的关系。方法:采用荧光免疫组化检测胃窦平滑肌细胞ghrelin受体(GHS-R)的表达;应用钙离子(Ca2+)指示剂Fluo-3/AM作为细胞内Ca2+的荧光探针,对负载培养的平滑肌细胞应用激光共聚焦显微镜技术,检测不同浓度ghrelin对平滑肌细胞内Ca2+荧光强度(FI)的影响,以及ghrelin受体阻断剂D-Lys3-GHRP-6、NO供体硝普钠(SNP),一氧化氮合酶(NOS)抑制剂N-硝基左旋精氨酸甲酯(L-NAME)对ghrelin调控Ca2+荧光强度的影响。结果:(1)豚鼠胃窦平滑肌细胞呈GHS-R免疫反应阳性表达.(2)随着ghrelin浓度升高(10-11,10-10,10-9,10-8,10-7mol/L),平滑肌细胞内Ca2+荧光强度逐渐升高,组间峰值(分别为54.7±11.5,58.1±5.7,64.8±6.6,84.9±7.1,95.7±10.5)和峰高(分别为1.8±0.3,2.1±0.8,5.3±1.3,28.9±4.2,37.6±3.7)均存在显著差异(P<0.05-0.01),即呈明显剂量依赖...
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| 关 键 词： | Ghrelin 胃窦平滑肌 钙离子 激光共聚焦 豚鼠 |
Study on the effects of Ghrelin on intracellular calcium mobilization and its relationship with NO in guinea pig gastric antrum smooth muscle cells |
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| XIN Fang-jie,XU Luo.Study on the effects of Ghrelin on intracellular calcium mobilization and its relationship with NO in guinea pig gastric antrum smooth muscle cells[J].Progress in Modern Biomedicine,2010,10(2). |
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| Authors: | XIN Fang-jie XU Luo |
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| Institution: | XIN Fang-jie,XU Luo(Department of Pathophysiology,Medical College of Qingdao University,Qingdao,Sh,ong,266021,China) |
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| Abstract: | Objective:To investigate the effects of ghrelin on intracellular Ca2+ mobilization in acutely isolated guinea pig gastric antrum smooth muscle cells and its ralationship with NO pathways. Methods:Expression of the Ghrelin receptor(GHR-S) was identified with immunofluorescence staining technique The effect of ghrelin on intracellular Ca2+ concentration of gastric antrum smooth muscle cells was detected by a laser confocal microscope using Fluo-3/AM as an the radiometric Ca2+ indicator. Furthermore,the effect... |
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| Keywords: | Ghrelin Gastric antrum smooth muscle cells Ca2+ confocal Guinea pig |
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