1. Institute of Electronic Structure and Laser, Foundation for Research and Technology, Heraklion, Crete, Greece;2. Medical School, University of Crete, Heraklion, Crete, Greece;3. Department of Biology, University of Crete, Heraklion, Crete, Greece
Abstract:
Third Harmonic Generation (THG) microscopy as a non‐invasive, label free imaging methodology, allows linkage of lipid profiles with various breast cancer cells. The collected THG signal arise mostly from the lipid droplets and the membrane lipid bilayer. Quantification of THG signal can accurately distinguish HER2‐positive cells. Further analysis using Fourier transform infrared (FTIR) spectra reveals cancer‐specific profiles, correlating lipid raft‐corresponding spectra to THG signal, associating thus THG to chemical information.
