A群脑膜炎球菌荚膜多糖纯化工艺的优化

目的对A群脑膜炎球菌荚膜多糖纯化工艺的关键步骤进行分步研究,优化每一步工艺参数。方法优化十六烷基三甲基溴化铵的加入浓度、复合多糖的解离浓度和解离时间、不同厂家的苯酚、超滤和透析等工艺过程对荚膜多糖的影响。结果十六烷基三甲基溴化铵质量体积终浓度0.10%(w/v)沉淀效果更好,纯化获得的荚膜多糖产量更高相对分子质量更大。复合多糖解离浓度越高,纯化获得的荚膜多糖相对分子质量越小。延长复合多糖解离时间有利于提高荚膜多糖产量。不同厂家的苯酚、超滤和透析等工艺对荚膜多糖的产量和分子大小没有影响。结论现行A群脑膜炎球菌荚膜多糖纯化工艺复杂,优化后的工艺提高了荚膜多糖产量,缩短了工艺用时,增加了工艺稳定性。

Optimization of purification process for capsular polysaccharide from group A Meningococcus

Objective Key processes of purification technology for capsular polysaccharide from group A Meningococcus were studied step by step so as to optimize parameters in process .Methods Final concentration of CTAB , dissociation time and concentration of compound polysaccharide , phenol of different manufactures and techniques used in ultrafiltration and dialysis were optimized .Results To obtain a minimum KD value and higher yield of polysaccharide ,the final concen-tration of CTAB with a 0.1%（w/v） is selected.Higher concentration for dissociation of compound polysaccharide makes a smaller molecular size for capsular polysaccharide .Extended dissociation time of compound polysaccharide is beneficial to improve the yield of capsular polysaccharide .Phenol from different manufactures and techniques used in ultrafiltration and dialysis make no differences to molecular size and yield of capsular polysaccharide .Conclusion There are many steps in the conventional purification process of capsular polysaccharide from group A Meningococcus.It is by optimized parameters in process of purification that the yield of capsular polysaccharide is improved, the time is shortened and consistence of process is increased .