Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures |
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| Authors: | Marcel A. K. Jansen Hilbert Booij Jan H. N. Schel Sacco C. de Vries |
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| Affiliation: | (1) Department of Molecular Biology, Agricultural University, Dreijenlaan 3, 6703, HA Wageningen, The Netherlands;(2) Present address: Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, 76100 Rehovot, Israel;(3) Department of Plant Cytology and Morphology, Agricultural University, Arboretumlaan 4, NL-6703 BD Wageningen, The Netherlands |
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| Abstract: | An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10–3 M were transferred to hormone-free medium containing 10–2 M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10–4 M calcium were transferred to hormone-free medium with 10–3 M calcium. At calcium concentrations between 6·10–3 and 10–2 M globular stage somatic embryos were found in cultures supplemented with 2·10–6 M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations. |
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| Keywords: | Calcium Daucus carota Somatic embryogenesis |
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