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Estimation of the quantity of bacteria encapsulated in Lentikats Biocatalyst via phospholipid fatty acids content: a preliminary study |
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| Authors: | Josef Trögl Ivana Jirková Petra Zemánková Věra Pila?ová Petra Dáňová Jana Pavlorková Pavel Kuráň Jan Popelka Lucie K?iklavová |
| Institution: | 1. Faculty of the Environment, Jan Evangelista Purkyně University, Králova Vy?ina 3132/7, 400 96, ústí nad Labem, Czech Republic 2. Research Institute of Inorganic Chemistry, a.s., Revolu?ní 84, 400 01, ústí nad Labem, Czech Republic 3. Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, Studentská 2, 461 17, Liberec, Czech Republic |
| Abstract: | The content of phospholipid fatty acids (PLFA) was determined in samples of polyvinyl alcohol lenses (Lentikats Biocatalyst, LB) with encapsulated Paracoccus denitrificans withdrawn during long-term denitrification experiments. The total PLFA content correlated highly with specific denitrification activities of LB as well as biomass estimation based on image analyses of microscopic photos. The results confirmed the applicability of PLFA determination for estimation of the amount of living encapsulated microbial biomass during biotechnological applications. |
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