Molecular specificity of 2-aminopurine in Saccharomyces cerevisiae |
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Authors: | S Sora L Panzeri G E Magni |
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Institution: | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, Warsaw Poland |
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Abstract: | The rates of DNA, RNA and protein synthesis were investigated by incorporation of radioactive precursors into the excised root tips of V. faba. 2-h exposure to 0.1% caffeine resulted in inhibition of protein synthesis to about 60% of the control rate. RNA synthesis was reduced in the range of 20–30%. The same concentration of caffeine did not affect the rate of DNA synthesis even during 12-h incubation, but concentrations higher than 1% caused a significant decrease in 3H]thymidine incorporation. |
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Keywords: | DEB diepoxybutane TCA tricholoroacetic acid |
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