A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus,Gigaspora margarita |
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| Authors: | Yokoyama Kazuhira Tateishi Takahiro Marumoto Takuya Saito Masanori |
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| Institution: | Department of Biological Science, Faculty of Agriculture, Yamaguchi University, 1677-1, Yoshida, Japan. wahei@agr.yamaguchi-u.ac.jp |
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| Abstract: | To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA. |
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| Keywords: | Gigaspora margarita Isolate-specific identification Diagnostic sequence Oligonucleotide probe |
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