沉默DEPDC1抑制鼻咽癌细胞HNE-1和CNE-1侵袭迁移

DEPDC1(DEP domain containing 1)是一个新的肿瘤相关基因，在多种恶性肿瘤的发生发展进程中起着重要作用。我们前期工作中在鼻咽癌细胞内沉默了DEPDC1的表达，发现抑制细胞增殖并诱发细胞凋亡。本研究旨在探讨沉默DEPDC1表达后，对鼻咽癌细胞HNE-1和CNE-1侵袭迁移能力的影响及其分子机制。结果显示，siRNA介导DEPDC1表达沉默后，细胞侧向运动能力、侵袭及迁移能力显著降低。qRT-PCR及Western印迹检测发现DEPDC1沉默导致EMT上游关键转录因子Twist1及间质细胞标志分子Vimentin表达显著下调。这些研究表明，鼻咽癌细胞中DEPDC1通过调节Twist1等EMT关键分子的表达在细胞侵袭转移过程中起关键作用。推测DEPDC1在鼻咽癌中高表达可能对于促进其侵袭转移具有重要作用，进而促进肿瘤发生发展，但具体分子机制仍有待更深入研究。

Silencing DEPDC1 Inhibits the Migration and Invasion of Nasopharyngeal Carcinoma HNE-1 and CNE-1 Cells

DEPDC1(DEP domain containing 1) is a novel tumor-related gene and plays important roles in the development of a variety of malignant tumors. Study indicated that DEPDC1-depletion repressed cell proliferation and induced apoptosis in HNE 1 nasopharyngeal carcinoma cells. This study aims to explore its role in cell migration and invasion.The results demonstrated that DEPDC1 depletion significantly inhibited the migration and invasion in HNE-1 and CNE-1 cells by wound healing and TransWell assays. The expression of Twist1 and Vimentin were down regulated as determined by qRT-PCR and Western blot. It suggested that high expression of DEPDC1 enhanced the migration and invasion in nasopharyngeal carcinoma, and the underlining molecular mechanism needed to be elucidated.