首页 | 本学科首页   官方微博 | 高级检索  
   检索      

重组酶聚合酶扩增技术:一种新的核酸扩增策略
引用本文:高威芳,朱鹏,黄海龙.重组酶聚合酶扩增技术:一种新的核酸扩增策略[J].中国生物化学与分子生物学报,2016,32(6):627-634.
作者姓名:高威芳  朱鹏  黄海龙
基金项目:国家出入境主要农产品快速检测关键技术研究与示范项目(No.2015C110018)和浙江省重大科技专项重点农业项目(No.2012C12009-4)
摘    要:重组酶聚合酶扩增 (recombinase polymerase amplification, RPA)是近年来兴起的一种等温核酸扩增技术,它比聚合酶链式反应(polymerase chain reaction, PCR)及其它等温扩增技术更快速、便捷、高效。本文将详细介绍RPA这项新颖的技术,并对其在医疗诊断、农业、食品、生物安全等方面的研究及应用进展进行综述。期望这项技术得到更多的关注,使其发展更加完善,将来在更多的领域充分发挥作用,甚至书写核酸检测历史新篇章。

关 键 词:重组酶聚合酶扩增  重组酶聚合酶扩增技术  等温核酸扩增  应用  
收稿时间:2015-09-14

Recombinase Polymerase Amplification: A New DNA/RNA Amplification Strategy
GAO Wei-Fang,ZHU Peng,HUANG Hai-Long.Recombinase Polymerase Amplification: A New DNA/RNA Amplification Strategy[J].Chinese Journal of Biochemistry and Molecular Biology,2016,32(6):627-634.
Authors:GAO Wei-Fang  ZHU Peng  HUANG Hai-Long
Abstract:Recombinase polymerase amplification(RPA) is a kind of isothermal nucleic acid amplification technique rising in recent years. RPA is more rapid, convenient and efficient than the polymerase chain reaction (PCR) and other isothermal amplification techniques. In this paper, this new technique will be introduced in detail, with reviewing over the development of its relevant researches and applications until now on in medical diagnosis, agriculture, food safety, biological safety and so on. It is expected that this technique will attract more attention and be perfect in the near future making it play an important role in more fields, and what’s more, write a new start in nucleic acid detection.
Keywords:recombinase polymerase amplification  recombinase polymerase amplification  isothermal nucleic acid amplification  application  
点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息
点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号