Stability of isoproterenol bound to cyanogen bromide-activated agarose. |
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Authors: | G Vanquelin M L Lacombe J Hanoune A D Strosberg |
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Institution: | 1. Laboratorium Chemie der Proteinen, V.U.B., St. Genesius Rode 1640, Belgium;2. Unité de Recherches INSERM U-99, Hôpital Henri Mondor, 94010 Créteil, France |
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Abstract: | The chemical stability and release of isoproterenol bound by diazotation to an insoluble agarose matrix has been investigated. It is demonstrated, by a double labeling procedure (3H]isoproterenol and 14C]-spacer arm), that the bound ligand is readily released in a soluble form. This occurs primarily through a chemical hydrolysis of the arm-linked ligand from the cyanogen bromide-activated agarose, and can be observed under extensive washing procedures as well as under more “physiological” incubation conditions. The instability of the agarose-arm linkage should lead to a critical analysis of physiological effects obtained with agarose bound hormones in vitro. |
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