一株葛根素羟基化真菌的筛选及鉴定

摘要：【目的】葛根素是一种常用的抗心血管药物，主要从葛根中提取，本文从土壤中分离转化葛根素的菌株，并对转化产物进行了鉴定。【方法】用马铃薯培养基从土壤中分离真菌，采用静息细胞转化法，通过高效液相色谱法检测转化效果，用有机溶剂萃取法和半制备高效液相色谱分离纯化得到转化产物，质谱和核磁共振分析转化产物。【结果】从土壤中分离到186株真菌，其中1株具有较高的转化活性，命名为NT-01，经形态学和ITS序列系统发育分析鉴定为粘帚霉菌（Gliocladium sp.）。质谱分析转化产物分子离子峰为433，而底物分子

Screening and identification of a fungal strain functioning in hydroxylation of puerarin

Objective]Puerarin is a Chinese herbal medicine.We isolated a fungus from soil which could convert puerarin to 3'-hydroxypuerarin.Methods]Strains were cultivated on potato dextrose agar(PDA).The biotransformation product was extracted with an organic solvent and detected on high performance liquid chromatography(HPLC).Results]A total of 186 fungal strains were isolated from soil,and strain NT-01was determined to transform puerarin into 3'-hydmxypuemfin.The product was identified as 3'-hydroxypuemrin by MS and 1H-NMR and ~(13)C-NMR.The morphology characteristic of strain NT-01 was consistent with Gliocladium deliguescens.Phylogenie analysis on sequences showed that strain NT-01 was clustered together with G. Deliguescens and G.viride with high boostrap supposing,so that strain NT-01 was identified as Gliocladium sp.Conclusion]Gtiocladium strain NT-01 can convert puerarin to 3'-hydroxypuerarin.