染料木黄酮对成骨细胞增殖分化的影响

目的：研究染料木黄酮对体外培养乳鼠颅盖骨成骨细胞增殖分化的影响。方法：取乳鼠颅盖骨,采用胶原-胰蛋白酶消化法,进行颅骨成骨细胞培养,取第二代成骨细胞,添加10^-5～10^-7mol／L染料木黄酮,在CO2孵箱中培养48h和72h后MTT比色法测定细胞增殖,培养72h采用^3H-TdR和^H-Pro掺入实验测定DNA和胶原合成。用试剂盒检测细胞裂解液碱性磷酸酶(ALP)活性。结果：染料木黄酮明显增加成骨细胞MTT的吸光度值、^3H-TdR和^3H-Pro的掺入,增加成骨细胞碱性磷酸酶活性。结论：染料木黄酮促进体外培养的乳鼠颅盖骨成骨细胞DNA和胶原的合成,促进增殖和分化。

Effects of genistein on proliferation and differentiation of osteoblast

Aim: To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures. Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein(10 -5 mol/L, 10 -6 mol/L and 10 -7 mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3 H -TdR and 3 H -proline incorporation. The activity of ALP were measured by ALP assay kit. Results: Genistein significantly increased osteoblast 3 H -TdR and 3 H -proline incorporation and MTT, 10 -6 mol/L genistein increased ALP activity. Conclution: Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures,and promoted osteoblast proliferation and differentiation.