Retention of Metabolic and Differentiation Potentials of Green Lavandula vera Callus after Freeze-Preservation |
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| Authors: | Watanabe Katsumi; Mitsuda Hisateru; Yamada Yasuyuki |
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| Institution: | 1Department of Agricultural Chemistry, Kyoto University Kyoto 606, Japan
2Interdisciplinary Research Institute of Environmental Sciences Kawaramachi Kojinguchi, Kyoto 602, Japan |
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| Abstract: | Green Lavandula vera callus that produced biotin was preservedsuccessfully in liquid nitrogen for up to 3 weeks. We foundthat the callus recovered after the freeze-preservation, retainingnot only the biosynthetic capability for biotin but also differentiationpotentials such as chloroplast development and plantlet formation.The significance of retention of the metabolic and differentiationpotentials of the callus is discussed in terms of the freeze-preservationof plant genetic resources.
* This study is dedicated to the late Professor J. Ashida. (Received September 1, 1982; Accepted November 5, 1982) |
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