女性阴道中产细菌素的乳杆菌筛选与鉴定

目的对从60例健康女性阴道中筛选出产生细菌素的优势乳杆菌进行鉴定，并为研制开发微生态制剂提供优良可靠菌种。方法利用牛津杯法筛选出19株乳杆菌，其菌株发酵乳酸量高并且产生细菌素。对19株乳杆菌进行了多项理化鉴定。结果19株乳杆菌分别为：格氏乳杆菌9株，唾液乳杆菌1株，卷曲乳杆菌9株。结论筛选的19株乳杆菌是健康女性阴道中的优势有益菌，具有较强的产酸能力，都产生细菌素，其中16株产生过氧化氢，某些菌株具有较高的生产应用价值。

Screening and identification the Lactobacillus producing bacteriocin in women vagina

Objective Identifying the dominant Lactobacillus producing bacteriocin,which were screened from 60 healthy women vaginas,provides choice and credible strains for the research of probiotic.Methods 19 strains of Lactobacillus which produce bacteriocin and abundant lactic acid were screened by the method of Oxford-cup tests,and they were identified by several important biochemical characteristics.Results According to the results of these experiments,19 strains were identified that 9 were Lactobacillus.gasseri,1 was Lactobacillus.salivarius and 9 were Lactobacillus.crispatus.Conclusions These 19 strains Lactobacillus which were screened through this experiment were dominant beneficial bacteria in the healthy female vagina,which had high ability of producing acid and bacteriocin.16 strains of them produce H_2O_2 and some had great probiotic production value.