Osmotic pressure and aggregate shape in BSA/poly(ethylene glycol)-lipid/Dextran solutions |
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Authors: | Castelletto Valeria Hamley Ian W Clifton Luke A Green Rebecca J |
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Institution: | School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, P.O. Box 226, Whiteknights, Reading, UK. V.Castelletto@reading.ac.uk |
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Abstract: | In this work we study the colloidal osmotic pressure (COP) and aggregate shape in phosphate saline buffer solutions (pH 7.4) containing bovine serum albumin (BSA), poly(ethylene glycol) lipid (PEG(2000)-PE) and Dextran (Dx). Dx was added to the BSA/PEG(2000)-PE system in order to increase the COP of the solution to levels comparable to the COP of healthy adults, with the aim of using the solution as a blood COP regulator. Dynamic light scattering and small angle X-ray scattering results shown the formation of BSA/PEG(2000)-PE/Dx aggregates in the solution. Osmometry results shown that the addition of Dx to the BSA/PEG(2000)-PE system could successfully increase the COP, through the formation of BSA/PEG(2000)-PE/Dx aggregates. The BSA/PEG(2000)-PE/Dx solutions attained COP=15 mm Hg, representing 60% of COP measured for healthy adults. |
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