The characterization and isolation of plant heterokaryons by flow cytometry |
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| Authors: | R. G. Alexander E. C. Cocking P. J. Jackson J. H. Jett |
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| Affiliation: | (1) Plant Genetic Manipulation Group, Department of Botany, Nottingham University, NG7 2RD, Nottingham, England;(2) Life Sciences Division, Los Alamos National Laboratory, MS M886 Los Alamos, New Mexico |
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| Abstract: | Summary Plant heterokaryons were identified and isolated from a population of protoplasts following fusion. Endogenous chlorophyll autofluorescence and exogenously supplied fluorochromes were utilized to differentiate between parental and heterokaryon populations. Flow cytometric analysis detected heterokaryons based upon the simultaneous presence of both chlorophyll and an exogenously supplied fluorochrome within one cell. These parameters were utilized to sort large numbers of heterokaryons from the fusion mixture using modified flow instrumentation. Modifications to the instrumentation which allowed this sorting are discussed. |
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| Keywords: | Flow cytometry Heterokaryon isolation Somatic hybridization Cell sorting |
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