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Study of a hydrophobic site on bovine alpha-lactalbumin by labeling with [125I]-TID |
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| Authors: | J C Van Ceunebroeck J Krebs I Hanssens F Van Cauwelaert |
| Abstract: | The hydrophobic, photoreactive probe 3-(trifluoromethyl)-3-(m-125I]iodophenyl) diazirine (125I]TID) labels apo-bovine alpha-lactalbumin but much less his Ca2+-form. The labeling of the apo-form is strong at protein concentrations of 0.5 mg ml-1 and increases with increasing concentration. Furthermore, increasing concentrations of NaCl, decrease the labeling of apo-alpha-lactalbumin with 125I]TID. |
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