Chilling-induced oxidative stress in young sal (Shorea robusta) seedlings |
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Authors: | S Keshavkant S C Naithani |
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Institution: | (1) Seed Biology Lab, School of Life Sciences, Pt. Ravishankar Shukla University, 492 010 Raipur, India |
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Abstract: | Aerial parts of the chilling-sensitive young sal seedlings showed overproduction of reactive oxygen species (ROS) and thiobarbituric
acid reactive substances (TBARS) in response to constant chilling exposure during November to March (9–14.1 °C) in field conditions.
Almost 4–6 fold increase in ROS was observed in aerial parts of chilling exposed seedlings than the control seedlings (maintained
in greenhouse). Increased formation of ROS was found to be closely associated with the rise in TBARS in leaf (5.8 fold) and
shoot (4.8 fold) tissues. On the contrary the leaf and shoot of control seedling and root of both control and chilling exposed
seedlings exhibited relatively very low levels of superoxide and TBARS. The chilling exposed seedlings also showed striking
weakening in the free radical processing enzyme systems. The low temperatures during November to March resulted in reduced
activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POX) and ascorbate peroxidase (APX) almost
by 49, 26, 7 and 78 % in leaves and 65, 46, 9 and 85% in shoots respectively compared to leaves and shoots of control seedlings.
Our results indicated that, substantially higher rates of liberation of superoxide and TBARS along with drastic failure of
antioxidant enzyme system in chilling sensitive sal seedlings leads to oxidative bursts terminating into irreversible injury
in leaves and shoot of these seedlings. |
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Keywords: | Shorea robusta chilling injury antioxidant enzymes lipid peroxidation superoxide radical |
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