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The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells |
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| Authors: | de Vries J Meier P Wackernagel W |
| Institution: | 1. Global Centre for Environmental Remediation, University of Newcastle, Australia;2. ICAR- National Research Centre on Plant Biotechnology, Pusa, New Delhi 110012, India;3. Future Industries Institute, University of South Australia, Australia;4. Formerly Department of Microbiology, Sri Krishnadevaraya University, Anantapur 515055, India;1. Education Scientific Center of Nanotechnology, Far Eastern Federal University, Vladivostok 690950, Russian Federation;2. Department of Biotechnology, Chonnam National University, Yeosu, Chonnam, 59626, Republic of Korea;3. Gene Expression and Therapy Group, King''s College London, Department of Medical and Molecular Genetics, School of Basic & Medical Biosciences, 8th Floor, Tower Wing, Guy''s Hospital, Great Maze Pond, London SE1 9RT, UK;4. Department of Toxicology and Forensics, School of Medicine, University of Crete, Heraklion, Crete, Greece;5. Pacific Institute of Geography, FEB RAS, Vladivostok 690041, Russian Federation |
| Abstract: | The nptII(+) gene present in the genome of transgenic potato plants transforms naturally competent cells of the soil bacteria Pseudomonas stutzeri and Acinetobacter BD413 (both harboring a plasmid with an nptII gene containing a small deletion) with the same high efficiency as nptII(+) genes on plasmid DNA (3x10(-5)-1x10(-4) transformants per nptII(+)) despite the presence of a more than 10(6)-fold excess of plant DNA. However, in the absence of homologous sequences in the recipient cells the transformation by nptII(+) dropped by at least about 10(8)-fold in P. stutzeri and 10(9)-fold in Acinetobacter resulting in the latter strain in < or =1x10(-13) transformants per nptII(+). This indicated a very low probability of non-homologous DNA fragments to be integrated by illegitimate recombination events during transformation. |
| Keywords: | Homologous recombination Illegitimate recombination Natural transformation Kanamycin resistance Acinetobacter Pseudomonas stutzeri |
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