Direct acid-catalysed transmethylation of lactobacillic acid in lactic acid bacteria |
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Authors: | Pekka A Nikkilä Tom S E Johnsson Simo V Laakso |
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Institution: | (1) Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, 02150 Espoo, Finland |
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Abstract: | Summary Cellular fatty acids inLactobacillus büchneri were transmethylated with H2SO4 catalyst in methanol at elevated temperature. By optimising the reaction time and the amounts of catalyst and methanol used at a fixed temperature it was possible to maximise the lactobacillic acid yield. The yield of lactobacillic acid with this method was better than with the traditional method using base-catalysed saponification followed by HCL-catalysed methylation. |
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