Studies on the growth ofThiobacillus ferrooxidans |
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Authors: | Dr Olli H Tuovinen Dr Donovan P Kelly |
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Institution: | (1) Department of Microbiology, Queen Elizabeth College, Atkins Building, Campden Hill, W8 7AH London, England;(2) Department of Agricultural Biochemistry Waite Agricultural Research Institute, The University of Adelaide, 5064 Glen Osmond, South Australia, Australia |
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Abstract: | Summary Uranyl sulphate (0.2–0.9 mM) inhibited ferrous iron oxidation by growing cultures ofThiobacillus ferrooxidans. The addition of 5–100 mM uranium to the cultures caused immediate cessation of carbon dioxide fixation, rapid loss of viability
and gradual depression of ferrous iron oxidation. Virtually no uranium was found in washed cells grown in the presence of
subtoxic to toxic amounts of uranyl sulphate. Uranium-poisoned organisms appeared plasmolyzed in electron micrographs. Cultures
tolerant to 5 mM UO2
2+ were develoepd by successive subculturing in increased uranium concentrations. The tolerance was maintained during subculturing
in uranium-free medium. Frequency of mutants resistant to 1.0 and 1.5 mM UO2
2+ was 1 per 1.3×106 and 1 per 9.0×108, respectively. The frequency was increased in the presence of 15–150 mM nickel, zinc and manganese. In liquid cultures, bivalent
cations and EDTA alleviated the toxicity of 2 mM uranyl sulphate. |
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Keywords: | Thiobacillus ferrooxidans Iron Thiosulphate Polythionates Bacterial Growth Colonies on Agar |
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