| 棉花抗逆相关基因GhDr1的克隆及生物信息学分析 |
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| 引用本文： | 丁忠涛,张锐,郭三堆.棉花抗逆相关基因GhDr1的克隆及生物信息学分析[J].生物技术通报,2011(1). |
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| 作者姓名： | 丁忠涛 张锐 郭三堆 |
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| 作者单位： | 中国农业科学院生物技术研究所,国家农作物基因资源与基因改良重大科学工程,北京,100081 |
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| 基金项目： | 农业部国家转基因重大专项(2008ZX005-004) |
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| 摘 要： | 通过TAIL-PCR的方法从棉花中克隆到未知功能的新基因GhDr1,生物信息学分析结果表明,GhDr1的预测蛋白含有酪氨酸激酶和蛋白酶C磷酸化位点、潜在的锌指类功能模块、MAPK磷酸化位点及MAPK相互作用识别位点;与GhDr1同源基因位于同一基因簇的基因多为参与逆境信号转导的蛋白。推测GhDr1基因可能在逆境信号转导途径的磷酸化级联反应中被调节,参与棉花逆境应答的生理过程。
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| 关 键 词： | 生物信息学 棉花 GhDr1基因 TAIL-PCR 抗逆 |
Cloning and Bioinformatics Analysis of Cotton Gene GhDr1 Related to Resistance to Abiotic Stresses |
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| Ding Zhongtao,Zhang Rui,Guo Sandui.Cloning and Bioinformatics Analysis of Cotton Gene GhDr1 Related to Resistance to Abiotic Stresses[J].Biotechnology Bulletin,2011(1). |
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| Authors: | Ding Zhongtao Zhang Rui Guo Sandui |
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| Institution: | Ding Zhongtao Zhang Rui Guo Sandui(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081) |
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| Abstract: | We cloned a new cotton gene GhDr1 of unknown function by the method of TAIL-PCR.The bioinformatics analysis results were as follows:phosphorylation sites of tyrosine kinase and protein C exist in the predicted protein sequence of GhDr1;potential zinc-finger functional motif,MAPK phosphorylation sites and MAPK interaction docking motif are also found in the predicted protein sequence of GhDr1;the genes located in the same gene cluster with the homologous genes of GhDr1 are mostly involved in signal transduct... |
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| Keywords: | Bioinformatics Cotton GhDr1 gene TAIL-PCR Resistance to abiotic stresses |
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