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| 淫羊藿苷促进宫颈癌TC-1细胞凋亡作用的研究 | |
| 引用本文： | 杜道兵,卢万根,曹丛仁,洪乐鹏,叶秉坤,许孟杰,郝彦利,冷水龙.淫羊藿苷促进宫颈癌TC-1细胞凋亡作用的研究[J].现代生物医学进展,2011,11(4):646-649. |
| 作者姓名： | 杜道兵 卢万根 曹丛仁 洪乐鹏 叶秉坤 许孟杰 郝彦利 冷水龙 |
| 作者单位： | 1. 广州医学院基础学院,广东,广州,510182 2. 江西省修水县第二人民医院,江西,九江,332400 3. 江西省修水县中医院,江西,九江,332400 |
| 基金项目： | 广东省自然科学基金No.07301608; 广州市教委科学基金No.08A036; 广州市科技局基金2007J1-C0231资助 |
| 摘 要： | 目的:研究淫羊藿苷体外对致宫颈癌TC-1细胞的增殖抑制及促凋亡作用。方法:利用细胞培养,用不同浓度的淫羊藿苷在一定的时间处理致宫颈癌TC-1细胞,光学显微镜直接观察药物对细胞的作用;MTT法检测淫羊藿苷对TC-1细胞的增殖抑制作用;Dapi核染色、Annexinv-FITC/PI流式细胞学检测细胞凋亡。结果:淫羊藿苷对TC-1细胞有显著的抑制作用,且呈时间、剂量依赖,20μg/ml作用72小时后,细胞抑制率达99%;DAPI核染色和流式细胞术检测可发现典型细胞凋亡特征。结论:淫羊藿苷对TC-1细胞增殖有抑制和促凋亡作用,并呈时间浓度依赖性。 |
| 关 键 词： | 淫羊藿苷 宫颈癌 TC-1细胞 细胞增殖 细胞凋亡 |
Effect of Icariin in Increasing Apoptosis of Cervical Cancer TC-1 Cells |
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| DU Dao-bing,LU Wan-gen,CAO Cong-ren,HONG Le-peng,YE Bing-kun,XU Meng-jie,HAO Yan-li LENG Shui-long.Effect of Icariin in Increasing Apoptosis of Cervical Cancer TC-1 Cells[J].Progress in Modern Biomedicine,2011,11(4):646-649. | |
| Authors: | DU Dao-bing LU Wan-gen CAO Cong-ren HONG Le-peng YE Bing-kun XU Meng-jie HAO Yan-li LENG Shui-long |
| Institution: | DU Dao-bing1,LU Wan-gen2,CAO Cong-ren3,HONG Le-peng1,YE Bing-kun1,XU Meng-jie1,HAO Yan-li1 LENG Shui-long1(1 Basic medical school,Guangzhou Medical College,Guangzhou 510182,China,2 The Second People's Hospita of Xiushui County,Jiangxi 332400,3 Xiushui County Hospital of Traditional Chinese Medicine,China) |
| Abstract: | Objective:To investigate the effect of icariin on proliferation and apoptosis.of TC-1 cells Methods:Based on the cul-ture of TC-1 cells in vitro,morphological changes of icariin-treated cells were detected by Optical microscope;The effect of icariin on growth of TC-1 cells was evaluated by MTT assay;DAPI stainin and flow cytometry(Annexin V-FITC/PI) were used to determine the apoptosis.Results:Optical microscope and MTT assay showed that the icariin had significant antiproliferative effect on TC-1 cells in ... |
| Keywords: | Icariin Cervical Cancer TC-1 cell Proliferation Apoptos |
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