| PCR相关技术在植物病毒检疫检测中的应用 |
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| 引用本文： | 顾青雷,刘昊,张绍红.PCR相关技术在植物病毒检疫检测中的应用[J].生物技术通报,2011(3). |
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| 作者姓名： | 顾青雷 刘昊 张绍红 |
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| 作者单位： | 张家港出入境检验检疫局,张家港,215600 |
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| 摘 要： | PCR和建立在PCR基础上的分子生物学技术以其灵敏、快速、简便等优点广泛应用于植物病毒的检测。阐述反转录聚合酶链式反应、免疫捕捉反转录PCR、PCR-单链构型多态性、实时荧光定量PCR、差异显示PCR和巢式PCR等相关技术的原理及其在植物病毒检测中的应用现状,以期为我国植物病毒的检疫检测提供有益参考。
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| 关 键 词： | 植物病毒 PCR 检疫检测 |
Application of PCR Related Techniques in Plant Virus Quarantine and Detection |
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| Gu Qinglei,Liu Hao,Zhang Shaohong.Application of PCR Related Techniques in Plant Virus Quarantine and Detection[J].Biotechnology Bulletin,2011(3). |
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| Authors: | Gu Qinglei Liu Hao Zhang Shaohong |
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| Institution: | Gu Qinglei Liu Hao Zhang Shaohong(Zhangjiagang Entry-Exit Inspection and Quarantion Bureau,Zhangjiagang 215600) |
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| Abstract: | PCR and the technology in molecular biology based on PCR have been widely used in the detection of plant virus for their high-sensitivity,fast-response and easily-operation.The paper elaborates the principles of technology of RT-PCR,IC-RT-PCR,PCR-SSCP,real-time PCR,DD-PCR and nested PCR and their application status in the detection of plant virus to provide reference for quarantine and detection of plant virus. |
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| Keywords: | Plant virus PCR Quarantine and detection |
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