Effect of Stress on the Ability of a phlA-Based Quantitative Competitive PCR Assay To Monitor Biocontrol Strain Pseudomonas fluorescens CHA0 |
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| Authors: | Fabio Rezzonico, Yvan Moë nne-Loccoz, Genevi ve D fago |
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| Affiliation: | Fabio Rezzonico, Yvan Moënne-Loccoz, and Geneviève Défago |
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| Abstract: | A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells. |
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