| DNA-EGS1386胞内诱导核酶P抑制人巨细胞病毒UL49基因的表达 |
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| 引用本文： | 崔延伟,曾志锋,李弘剑,李月琴,周琪,杨丹,邹奕,杨光,周天鸿.DNA-EGS1386胞内诱导核酶P抑制人巨细胞病毒UL49基因的表达[J].生物工程学报,2009,25(11):1690-1696. |
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| 作者姓名： | 崔延伟 曾志锋 李弘剑 李月琴 周琪 杨丹 邹奕 杨光 周天鸿 |
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| 作者单位： | 暨南大学生命科学技术学院基因工程药物国家工程研究中心,广州,510632 |
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| 基金项目： | 国家自然科学基金(Nos.90608024,30370776);;广州市科技攻关项目(No.2006J1-C0111);;广东省科技计划项目(No.2006B35502002);;广东省自然科学基金重点项目(No.36703);;中国博士后科学基金资助项目(No.20080430845)资助~~ |
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| 摘 要： | 外部引导序列(EGSs)是一类与mRNA靶序列互补并能引导核酶P切割靶mRNA的小分子RNA。本实验构建稳定表达UL49基因的HeLa细胞系,设计合成了针对于人巨细胞病毒(HCMV)UL49基因的12ntDNA性质的EGS1386,通过转染稳定表达UL49基因的细胞系,荧光定量PCR和Western blotting检测细胞内目的基因UL49的表达情况。结果显示在DNA-EGS1386作用下UL49基因的表达量降低了50%,表明DNA-EGS1386可以有效引导人的核酶P切割目标mRNA。因此,DNA-EGS可以发展成为一种新的基因沉默技术和潜在的抗病毒试剂。
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| 关 键 词： | 短的EGS 基因沉默 UL49基因 核酶P |
| 收稿时间： | 2009/7/12 0:00:00 |
DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene |
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| Yanwei Cui,Zhifeng Zeng,Hongjian Li,Yueqin Li,Qi Zhou,Dan Yang,Yi Zou,Guang Yang and Tianhong Zhou.DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene[J].Chinese Journal of Biotechnology,2009,25(11):1690-1696. |
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| Authors: | Yanwei Cui Zhifeng Zeng Hongjian Li Yueqin Li Qi Zhou Dan Yang Yi Zou Guang Yang and Tianhong Zhou |
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| Institution: | National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China;National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, China |
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| Abstract: | External Guide Sequences(EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression.They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA.DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus(HCMV).The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target... |
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| Keywords: | miniEGSs gene silencing UL49 gene RNase P |
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