The Micronuclear Gene Encoding β-Telomere Binding Protein in Oxytricha nova |
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Authors: | JENNIFER L MITCHAM DAVID M PRESCOTT MICHAEL K MILLER |
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Institution: | Immunex Research and Development Corporation, 51 University Street, Seattle, Washington 98101;Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347 |
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Abstract: | ABSTRACT The micronuclear version of the gene encoding β-telomere binding protein (β-TBP) in Oxytricha nova has been sequenced and compared to the macronuclear β-TBP gene, previously described. The micronuclear gene contains three AT-rich internal eliminated sequences (IES) of 37, 40, and 43 bp and four macronuclear destined sequences (MDS). The IES interrupt the gene once near the 5′ end of the coding region and twice in the 3′ trailer downstream from the TGA stop codon. The sequences of the micronuclear and macronuclear genes are colinear. Thus, the micronuclear β-TBP gene is not scrambled, which contrasts with the highly scrambled state among the 14 MDS in the micronuclear α;-TBP gene. |
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Keywords: | Gene scrambling internal eliminated sequences (IES) macronuclear development |
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