Evaluation of extracellular lipid peroxidation in brain cortex of anaesthetized rats by microdialysis perfusion and high-performance liquid chromatography with fluorimetric detection |
| |
Authors: | Chung-Shi Yang Pi-Ju Tsai Jen-Pey Wu Nai-Nu Lin Su-Tze Chou Jon-Son Kuo |
| |
Institution: | aDepartment of Medical Research, Taichung Veterans General Hospital, Taichung 40705, Taiwan;bDepartment of Food and Nutrition, Providence University, Taichung 40705, Taiwan |
| |
Abstract: | A method for in vivo evaluation of lipid peroxidation in the extracellular space of anaesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion and high-performance liquid chromatography. The microdialysates, eluted from implanted probes, were reacted with thiobarbituric acid (TBA) prior to analysis by an HPLC system equipped with a fluorescence detector (excitation and emission wavelengths were 515 and 550 nm, respectively). Lipid peroxidation in the extracellular space was evaluated as the concentration of malondialdehyde, a lipid peroxidation end product which reacts with TBA to form a fluorescent conjugate. Significantly increased production of malondialdehyde following hydrogen peroxide perfusion (0.03%, 0.3% at a flow-rate of 1 μl/min) was observed in the brain cortex of anaesthetized rats. |
| |
Keywords: | Lipids Malondialdehyde Thiobarbituric acid |
本文献已被 ScienceDirect 等数据库收录! |
|