Rapid isolation of lysyl-tRNA synthetase from rat liver |
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| Authors: | A M Kumar R Nayak |
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| Institution: | Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore. |
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| Abstract: | The high molecular weight aminoacyl-tRNA synthetase complex (the 24S complex) was isolated from rat liver by ultracentrifugation. The lysyl-tRNA synthetase (E.C. 6.1.1.6) was selectively dissociated by hydrophobic interaction chromatography on 1,6 diaminohexyl agarose followed by hydroxylapatite chromatography and DEAE chromatography. The lysyl-tRNA synthetase dissociated from the 24S synthetase complex was purified approximately to 2700 fold with 14% yield. |
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