An 8 kb Nucleotide Sequence at the 3' Flanking Region of the sspC Gene (184{degrees}) on the Bacillus subtilis 168 Chromosome Containing an Intein and an Intron |
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Authors: | Ghim Sa-Youl; Choi Soo-Keun; Shin Byung-Sik; Park Seung-Hwan |
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Institution: | Bacterial Molecular Genetics RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB) P.O. Box 115, Yusong, Taejon 305-600, Korea |
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Abstract: | As part of the Bacillus subtilis genome sequencing project,we determined the complete nucleotide sequence of an 8000-bpfragment downstream of the sspC gene (184°) of the B. subtilis168 chromosome. The sequence analysis shows that the sspC geneis located inside of the SPß region, which differsfrom the current genetic map of B. subtilis 168. This regioncontains 12 putative ORFs (yojQ through yojZ and sspC). A homologysearch for the deduced products of the ORFs shows signi.cantsimilarities to enzymes involved in deoxyribonucleotide metabolism:ribonucleotide reductase (Nrd) E, NrdF, thioredoxinand dUTPase.Interestingly, this DNA fragment includes two split genes, yojPcontaining conserved motifs of an intein and yojQ and yojS withan 808-bp intervening sequence for a putative intron structure.In addition, the yojR gene includes a putative new DNA replicationterminator. |
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Keywords: | Bacillus subtilis genome sequencing yojP-sspC region intein intron |
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