PHENOL OXIDASE ACTIVITY IN ANOPHELES SINENSIS AND AEDES ALBOPZCTUS EXPOSED TO MICROFILARIAE OF BRUGIA MALAYI |
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Authors: | Xiaojun Pan Ping Jin |
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Institution: | Department of Biology, Guangzhou Normal College;, Guangzhou 510400, China Department of Parasitology, Gwngzhou Medical College;, Guangzhou 510182, China |
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Abstract: | Abstract Phenol oxidase (PO activity was investigated in cell-free hemolymph collected from Anopheles sinensis and Aedes albopictlcs female adults intrathoracically inoculated with Brugia dayi microfilariae (mff), inoculated with saline alone, or from uninoculated mosquitoes. The activity of PO from uninoculated 1-day-old mosquitoes was significantly greater than those of mosquitoes on 3, 12 and 16 day. PO activity in mff-inoculated A. sinensis at 24 h postinoculation (PI) was 2–3 times higher as compared with uninoculated or saline-inoculated mosquitoes. Inoculation of B. malayi mff into A. albopictus elicited ?-fold increase in PO activity at 24 h PI as compared with uninoculated mosquitoes. Immune-activated levels of PO activity in A. alboplctus was significantly higher as compared with those seen in A. sinensis. The relationship of observed differences in PO activity to differences in immunological capability between A. sinensis and A. albopictus is discussed. |
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Keywords: | Anopheles sinensis Aedes albopictus Brugia dayi phenol oxidase |
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