| 高效微生物絮凝剂产生菌TJ-3的絮凝特性分析 |
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| 引用本文： | 宁 雪,常玉广,丁西明,夏四清.高效微生物絮凝剂产生菌TJ-3的絮凝特性分析[J].微生物学通报,2009,36(5):0640-0643. |
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| 作者姓名： | 宁 雪 常玉广 丁西明 夏四清 |
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| 作者单位： | 同济大学,污染控制与资源化研究国家重点实验室,上海,200092 |
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| 基金项目： | 中国博士后基金(No. 20070420671); 上海市科委世博科技专项(No. 07DZ05814); 中俄科技合作项目(No. 2007DFR90050) |
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| 摘 要： | 从活性污泥中分离筛选出一株高效絮凝剂产生菌TJ-3, 经生理生化试验检测其属于革兰氏阴性菌, 短杆状, 16S rDNA测序鉴定为铜绿假单胞菌。生长曲线表明, TJ-3的生长稳定期较长, 所产微生物絮凝剂(MBF)的稳定性良好。TJ-3产MBF对高岭土悬液具有良好的絮凝效果, 最佳条件下的絮凝率为98.2%。絮凝活性分布实验结果表明, TJ-3所产MBF的活性物质大部分存在于离心后的沉淀物中。处理100 mL高岭土悬液, pH值8.5、1%(质量分数)CaCl2溶液投加量3.5 mL、菌液投加量1.5 mL时, 絮凝效果最佳。
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| 关 键 词： | 微生物絮凝剂 絮凝率 高岭土悬液 生长曲线 16S rDNA |
Analysis on Flocculation Characteristics of Bioflocculant-producting Bacteria TJ-3 |
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| NING Xue,CHANG Yu-Guang,DING Xi-Ming and XIA Si-Qing.Analysis on Flocculation Characteristics of Bioflocculant-producting Bacteria TJ-3[J].Microbiology,2009,36(5):0640-0643. |
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| Authors: | NING Xue CHANG Yu-Guang DING Xi-Ming and XIA Si-Qing |
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| Institution: | State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China;State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China;State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China;State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China |
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| Abstract: | A strain of high-efficiency bioflocculant-producting bacteria, which was named TJ-3, was screened from sewage sludge. The strain was gram-negative and rod-shaped in physiological biochemical test and was identified as Pseudomonas aeruginosa by 16S rDNA Sequencing. According to the growth curve of the TJ-3 strain, the growth stabilization period is long so that Microbial Flocculants (MBF) production is stable. The MBF produced by the TJ-3 strain is able to flocculante kaolin suspension with 98.2%. The MBF activity distribution tests show that most of the active components of the bioflocculant exist in deposition after centrifufation. When pH is 8.5, 1% (quality fraction) CaCl2 Solution dosing quantity is 3.5 mL, bioflocculant dosing quantity is 1.5 mL in 100 mL kaolin suspension, the bioflocculant has an optimal flocculation. |
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| Keywords: | Bioflocculant Flocculating rate Kaolin suspension Growth curve 16S rDNA |
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