| 叶绿体转化三角褐指藻表达外源蛋白 |
| |
| 引用本文： | 朱聪聪,张乃升,谢伟红,牛莹芳,杨维东,刘洁生,李宏业.叶绿体转化三角褐指藻表达外源蛋白[J].热带亚热带植物学报,2011,19(3):267-272. |
| |
| 作者姓名： | 朱聪聪 张乃升 谢伟红 牛莹芳 杨维东 刘洁生 李宏业 |
| |
| 作者单位： | 1. 暨南大学生物工程学系,广州,510632
2. 暨南大学生物工程学系,广州510632;暨南大学广东省高校水体富营养化与赤潮防治重点实验室,广州510632 |
| |
| 基金项目： | 广东省科技计划项目(2009B020301002,2009B050600005, 2010B030600005)；中央高校基本科研业务费专项基金(21610103) |
| |
| 摘 要： | 为建立三角褐指藻(Phaeodacty lum tricornutum)叶绿体表达体系,从其叶绿体基因组中克隆了rnS-trnI、trnAml序列作为遗传转化同源重组序列,并以氯霉素抗性基因(CAT)表达盒作为筛选标记,以及绿色荧光蛋白基因(GFP)表达盒作为报告基因.以TA克隆载体pMD19-T为基础,将CAT表达盒...
|
| 关 键 词： | 三角褐指藻 叶绿体转化 同源重组 电穿孔法 |
| 收稿时间： | 2011/2/20 0:00:00 |
| 修稿时间： | 2011/3/29 0:00:00 |
Foreign Protein Expression in Chloroplast Transformation of Phaeodactylum tricornutum |
| |
| ZHU Cong-cong,ZHANG Nai-sheng,XIE Wei-hong,NIU Ying-fang,YANG Wei-dong,LIU Jie-sheng and LI Hong-ye.Foreign Protein Expression in Chloroplast Transformation of Phaeodactylum tricornutum[J].Journal of Tropical and Subtropical Botany,2011,19(3):267-272. |
| |
| Authors: | ZHU Cong-cong ZHANG Nai-sheng XIE Wei-hong NIU Ying-fang YANG Wei-dong LIU Jie-sheng and LI Hong-ye |
| |
| Institution: | College of Biological Engineering, Jinan University,College of Biological Engineering, Jinan University,College of Biological Engineering, Jinan University,College of Biological Engineering, Jinan University,Department of Biotechnology; Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher EducationInstitutes, Jinan University,Department of Biotechnology; Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher EducationInstitutes, Jinan University,Department of Biotechnology; Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher EducationInstitutes, Jinan University |
| |
| Abstract: | To achieve foreign protein expression in the chloroplast of Phaeodactylum tricornutum, a chloroplast transformation vector was constructed. The sequences rns-trnI and trnA-rnl from P. tricornutum chloroplast genome were cloned and used as homologous recombination elements. Chloramphenicol acetyltransferase gene (CAT) expression cassette conferring chloramphenicol resistance was employed as selection marker, and green fluorescent protein gene (GFP) as reporter gene. Based on the TA cloning vector pMD19-T, CAT and GFP expression cassette were cloned in between the two homologous recombination elements. The resultant chloroplast transformation vector was transferred into the chloroplast of P. tricornutum by electroporation method. Transplastomic P. tricornutum cells were obtained upon chloramphenicol selection and reporter GFP could be detected in the chloroplasts, indicating that chloroplast expression system was successfully achieved. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《热带亚热带植物学报》浏览原始摘要信息 |
| 点击此处可从《热带亚热带植物学报》下载免费的PDF全文 |
|
